| Titre : | BACTERIAL MENINGITIS IN A MOROCCAN POPULATION : CASES OF NEISSERIA MENINGITIDIS, STREPTOCOCCUS PNEUMONIAE AND HAEMOPHILUS INFLUENZAE PHENOTYPIC AND GENOTYPIC CHARACTERIZATION | | Type de document : | thèse | | Auteurs : | IKKEN Youssef, Auteur | | Année de publication : | 2022 | | Langues : | Anglais (eng) | | Mots-clés : | Antibioresistance Bacterial meningitis Biphasic medium CSF-Culture Molecular epidemiology Quantitative PCR Vaccines Antibioresistance Culture de LCR Epidémiologie moléculaire Méningite
bactérienne Milieu biphasique PCR quantitative vaccins المقاومة للمضادات الحیویة للمیكروبات التھاب السحایا الجرثومي وسط غذائي ثنائي الطور، علم
الأوبئة الجزیئیة؛ في الاستزراع الجرثومي السلبي للسائل الدماغي الشوكي تفاعل البلمرة المتسلسل الكمي والمتعدد مصل
/لقاح. | | Résumé : | Objective : The aim of the study is to provide updated epidemiology, antibioresistance profile,
phenotypic and molecular characterization of bacterial meningitis circulated in the Moroccan
population. Materials and methods : The study conducted from 2012 to 2018, covering the
most of the Moroccan territory. The CSF samples and the strains isolated from these CSFs were
included. Data analysis was anonymized, collected in Excel and processed by Epi Info and
SPSS software. Results : The 1st study investigated 16 S. pneumoniae and found 9 serotypes
with different resistance to PG and other antibiotic families. The 2nd study, altogether 183
isolates were collected and N. meningitidis was predominant, followed by S. pneumoniae and
H. influenzae type b. The N. meningitidis serogroup B was predominant followed by serogroup
W135. The decreased susceptibility to Penicillin G (DSPG) for all isolates accounted for 15.7%
and other resistances were observed. The 3rd study, 90 CSFs were collected and showed that
the confirmation rate per culture recorded a rate of 33% and the reel time PCR of 70%. Also,
the molecular epidemiology confirmed the predominance of N. meningitidis followed by S.
pneumoniae and H. influenzae. And the results of the 4th study showed that the biphasic medium
provided more, novels and easy nutriments through the addition of liquid phase and solid phase
medium and it was found to be conducive to the growth and conservation of N. meningitidis, S.
pneumoniae and H. influenzae at an ambient temperature of a minimum of 40 days.
Conclusion : The three isolates were highly susceptible to antibiotics. And the vaccines against
H. influenzae type b and S. pneumoniae reduced the meningitis cases due to these two species.
The PCR combined with CSF-culture improve the laboratory diagnosis, and reinforce the
epidemiological surveillance. And the biphasic medium could be used as a technical solution
for the better management of meningitis in Morocco. | | Numéro (Thèse ou Mémoire) : | D0292021 | | Président : | SEKHSOKH YASSINE | | Directeur : | ZOUHDI MIMOUN | | Juge : | CHADLI MARIAMA | | Juge : | EL ANNAZ HICHAM | | Juge : | CHAROF REDA |
BACTERIAL MENINGITIS IN A MOROCCAN POPULATION : CASES OF NEISSERIA MENINGITIDIS, STREPTOCOCCUS PNEUMONIAE AND HAEMOPHILUS INFLUENZAE PHENOTYPIC AND GENOTYPIC CHARACTERIZATION [thèse] / IKKEN Youssef, Auteur . - 2022. Langues : Anglais ( eng) | Mots-clés : | Antibioresistance Bacterial meningitis Biphasic medium CSF-Culture Molecular epidemiology Quantitative PCR Vaccines Antibioresistance Culture de LCR Epidémiologie moléculaire Méningite
bactérienne Milieu biphasique PCR quantitative vaccins المقاومة للمضادات الحیویة للمیكروبات التھاب السحایا الجرثومي وسط غذائي ثنائي الطور، علم
الأوبئة الجزیئیة؛ في الاستزراع الجرثومي السلبي للسائل الدماغي الشوكي تفاعل البلمرة المتسلسل الكمي والمتعدد مصل
/لقاح. | | Résumé : | Objective : The aim of the study is to provide updated epidemiology, antibioresistance profile,
phenotypic and molecular characterization of bacterial meningitis circulated in the Moroccan
population. Materials and methods : The study conducted from 2012 to 2018, covering the
most of the Moroccan territory. The CSF samples and the strains isolated from these CSFs were
included. Data analysis was anonymized, collected in Excel and processed by Epi Info and
SPSS software. Results : The 1st study investigated 16 S. pneumoniae and found 9 serotypes
with different resistance to PG and other antibiotic families. The 2nd study, altogether 183
isolates were collected and N. meningitidis was predominant, followed by S. pneumoniae and
H. influenzae type b. The N. meningitidis serogroup B was predominant followed by serogroup
W135. The decreased susceptibility to Penicillin G (DSPG) for all isolates accounted for 15.7%
and other resistances were observed. The 3rd study, 90 CSFs were collected and showed that
the confirmation rate per culture recorded a rate of 33% and the reel time PCR of 70%. Also,
the molecular epidemiology confirmed the predominance of N. meningitidis followed by S.
pneumoniae and H. influenzae. And the results of the 4th study showed that the biphasic medium
provided more, novels and easy nutriments through the addition of liquid phase and solid phase
medium and it was found to be conducive to the growth and conservation of N. meningitidis, S.
pneumoniae and H. influenzae at an ambient temperature of a minimum of 40 days.
Conclusion : The three isolates were highly susceptible to antibiotics. And the vaccines against
H. influenzae type b and S. pneumoniae reduced the meningitis cases due to these two species.
The PCR combined with CSF-culture improve the laboratory diagnosis, and reinforce the
epidemiological surveillance. And the biphasic medium could be used as a technical solution
for the better management of meningitis in Morocco. | | Numéro (Thèse ou Mémoire) : | D0292021 | | Président : | SEKHSOKH YASSINE | | Directeur : | ZOUHDI MIMOUN | | Juge : | CHADLI MARIAMA | | Juge : | EL ANNAZ HICHAM | | Juge : | CHAROF REDA |
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